Webdocument 2.1. Myoglobin has three accession numbers corresponding to three splice variants. The DNA entries are as follows:

 
1: NM_005368
Homo sapiens myoglobin (MB), transcript variant 1, mRNA
gi|44955876|ref|NM_005368.2|[44955876]
 
2: NM_203377
Homo sapiens myoglobin (MB), transcript variant 2, mRNA
gi|44955884|ref|NM_203377.1|[44955884]
 
3: NM_203378
Homo sapiens myoglobin (MB), transcript variant 3, mRNA
gi|44955887|ref|NM_203378.1|[44955887]

The alignment of the DNA is as follows. To do this, enter the query “NM_005368, NM_203377, NM_203378” into Entrez Nucleotide, select the FASTA format, send to text, and then use a program such as MUSCLE (Chapter 6) to perform a multiple sequence alignment.. This shows that only the 5’ ends of the sequences vary. The start ATG is highlighted.

MUSCLE (3.6) multiple sequence alignment
 
 
gi|44955887|ref|NM_203378.1| -----AATGGCACCTGCCCTAAAATAGCTTCCCATGTGAGGGCTAGAGAAAGGAAA----
gi|44955876|ref|NM_005368.2| ------GCAGCCTCAAACC-----------------------------------------
gi|44955884|ref|NM_203377.1| GAGCATGTTGGCCTGGTCCTTTGCTAGGTACTGTAGAGCAGGTGAGAGAGTGAGGGGGAA
 * ** 
 
gi|44955887|ref|NM_203378.1| -------AGATTAGACCCTCCCT---GGATGAGAGAGAGAAAGTGAAGGAGGGCAGGGGA
gi|44955876|ref|NM_005368.2| ---------------CCAGCTGTT------------------------GGGGCCAGGACA
gi|44955884|ref|NM_203377.1| GGACTCCAAATTAGACCAGTTCTTAGCCATGAAGCAGAGACTCT----GAAGCCAGACTA
  ** * * * *** *
 
gi|44955887|ref|NM_203378.1| GGGGGACAGCGAGCCATTGAGC----GATCTTTGTCAAGCATCCCAGAAGACTGCGCCAT
gi|44955876|ref|NM_005368.2| CCCAGTGAGCCCATACTTGCTCTTTTTGTCTTCTTC------------AGACTGCGCCAT
gi|44955884|ref|NM_203377.1| CCTGGGT--CCCAATCTTGGGCTTGGTATTTCCTCGCTGTGTGACTCTGGACTGCGCCAT
 * * *** * * * ***********
 
gi|44955887|ref|NM_203378.1| GGGGCTCAGCGACGGGGAATGGCAGTTGGTGCTGAACGTCTGGGGGAAGGTGGAGGCTGA
gi|44955876|ref|NM_005368.2| GGGGCTCAGCGACGGGGAATGGCAGTTGGTGCTGAACGTCTGGGGGAAGGTGGAGGCTGA
gi|44955884|ref|NM_203377.1| GGGGCTCAGCGACGGGGAATGGCAGTTGGTGCTGAACGTCTGGGGGAAGGTGGAGGCTGA
  ************************************************************
 
gi|44955887|ref|NM_203378.1| CATCCCAGGCCATGGGCAGGAAGTCCTCATCAGGCTCTTTAAGGGTCACCCAGAGACTCT
gi|44955876|ref|NM_005368.2| CATCCCAGGCCATGGGCAGGAAGTCCTCATCAGGCTCTTTAAGGGTCACCCAGAGACTCT
gi|44955884|ref|NM_203377.1| CATCCCAGGCCATGGGCAGGAAGTCCTCATCAGGCTCTTTAAGGGTCACCCAGAGACTCT
 ************************************************************
 
gi|44955887|ref|NM_203378.1| GGAGAAGTTTGACAAGTTCAAGCACCTGAAGTCAGAGGACGAGATGAAGGCGTCTGAGGA
gi|44955876|ref|NM_005368.2| GGAGAAGTTTGACAAGTTCAAGCACCTGAAGTCAGAGGACGAGATGAAGGCGTCTGAGGA
gi|44955884|ref|NM_203377.1| GGAGAAGTTTGACAAGTTCAAGCACCTGAAGTCAGAGGACGAGATGAAGGCGTCTGAGGA
 ************************************************************
 
gi|44955887|ref|NM_203378.1| CTTAAAGAAGCATGGTGCCACCGTGCTCACCGCCCTGGGTGGCATCCTTAAGAAGAAGGG
gi|44955876|ref|NM_005368.2| CTTAAAGAAGCATGGTGCCACCGTGCTCACCGCCCTGGGTGGCATCCTTAAGAAGAAGGG
gi|44955884|ref|NM_203377.1| CTTAAAGAAGCATGGTGCCACCGTGCTCACCGCCCTGGGTGGCATCCTTAAGAAGAAGGG
 ************************************************************
 
gi|44955887|ref|NM_203378.1| GCATCATGAGGCAGAGATTAAGCCCCTGGCACAGTCGCATGCCACCAAGCACAAGATCCC
gi|44955876|ref|NM_005368.2| GCATCATGAGGCAGAGATTAAGCCCCTGGCACAGTCGCATGCCACCAAGCACAAGATCCC
gi|44955884|ref|NM_203377.1| GCATCATGAGGCAGAGATTAAGCCCCTGGCACAGTCGCATGCCACCAAGCACAAGATCCC
 ************************************************************
 
gi|44955887|ref|NM_203378.1| CGTGAAGTACCTGGAGTTCATCTCGGAATGCATCATCCAGGTTCTGCAGAGCAAGCATCC
gi|44955876|ref|NM_005368.2| CGTGAAGTACCTGGAGTTCATCTCGGAATGCATCATCCAGGTTCTGCAGAGCAAGCATCC
gi|44955884|ref|NM_203377.1| CGTGAAGTACCTGGAGTTCATCTCGGAATGCATCATCCAGGTTCTGCAGAGCAAGCATCC
 ************************************************************
 
gi|44955887|ref|NM_203378.1| CGGGGACTTTGGTGCTGATGCCCAGGGGGCCATGAACAAGGCCCTGGAGCTGTTCCGGAA
gi|44955876|ref|NM_005368.2| CGGGGACTTTGGTGCTGATGCCCAGGGGGCCATGAACAAGGCCCTGGAGCTGTTCCGGAA
gi|44955884|ref|NM_203377.1| CGGGGACTTTGGTGCTGATGCCCAGGGGGCCATGAACAAGGCCCTGGAGCTGTTCCGGAA
 ************************************************************
 
gi|44955887|ref|NM_203378.1| GGACATGGCCTCCAACTACAAGGAGCTGGGCTTCCAGGGCTAGGCCCCTGCCGCTCCCAC
gi|44955876|ref|NM_005368.2| GGACATGGCCTCCAACTACAAGGAGCTGGGCTTCCAGGGCTAGGCCCCTGCCGCTCCCAC
gi|44955884|ref|NM_203377.1| GGACATGGCCTCCAACTACAAGGAGCTGGGCTTCCAGGGCTAGGCCCCTGCCGCTCCCAC
 ************************************************************
 
gi|44955887|ref|NM_203378.1| CCCCACCCATCTGGGCCCCGGGTTCAAGAGAGAGCGGGGTCTGATCTCGTGTAGCCATAT
gi|44955876|ref|NM_005368.2| CCCCACCCATCTGGGCCCCGGGTTCAAGAGAGAGCGGGGTCTGATCTCGTGTAGCCATAT
gi|44955884|ref|NM_203377.1| CCCCACCCATCTGGGCCCCGGGTTCAAGAGAGAGCGGGGTCTGATCTCGTGTAGCCATAT
 ************************************************************
 
gi|44955887|ref|NM_203378.1| AGAGTTTGCTTCTGAGTGTCTGCTTTGTTTAGTAGAGGTGGGCAGGAGGAGCTGAGGGGC
gi|44955876|ref|NM_005368.2| AGAGTTTGCTTCTGAGTGTCTGCTTTGTTTAGTAGAGGTGGGCAGGAGGAGCTGAGGGGC
gi|44955884|ref|NM_203377.1| AGAGTTTGCTTCTGAGTGTCTGCTTTGTTTAGTAGAGGTGGGCAGGAGGAGCTGAGGGGC
 ************************************************************
 
gi|44955887|ref|NM_203378.1| TGGGGCTGGGGTGTTGAAGTTGGCTTTGCATGCCCAGCGATGCGCCTCCCTGTGGGATGT
gi|44955876|ref|NM_005368.2| TGGGGCTGGGGTGTTGAAGTTGGCTTTGCATGCCCAGCGATGCGCCTCCCTGTGGGATGT
gi|44955884|ref|NM_203377.1| TGGGGCTGGGGTGTTGAAGTTGGCTTTGCATGCCCAGCGATGCGCCTCCCTGTGGGATGT
 ************************************************************
 
gi|44955887|ref|NM_203378.1| CATCACCCTGGGAACCGGGAGTGGCCCTTGGCTCACTGTGTTCTGCATGGTTTGGATCTG
gi|44955876|ref|NM_005368.2| CATCACCCTGGGAACCGGGAGTGGCCCTTGGCTCACTGTGTTCTGCATGGTTTGGATCTG
gi|44955884|ref|NM_203377.1|  CATCACCCTGGGAACCGGGAGTGGCCCTTGGCTCACTGTGTTCTGCATGGTTTGGATCTG
 ************************************************************
 
gi|44955887|ref|NM_203378.1| AATTAATTGTCCTTTCTTCTAAATCCCAACCGAACTTCTTCCAACCTCCAAACTGGCTGT
gi|44955876|ref|NM_005368.2| AATTAATTGTCCTTTCTTCTAAATCCCAACCGAACTTCTTCCAACCTCCAAACTGGCTGT
gi|44955884|ref|NM_203377.1| AATTAATTGTCCTTTCTTCTAAATCCCAACCGAACTTCTTCCAACCTCCAAACTGGCTGT
 ************************************************************
 
gi|44955887|ref|NM_203378.1| AACCCCAAATCCAAGCCATTAACTACACCTGACAGTAGCAATTGTCTGATTAATCACTGG
gi|44955876|ref|NM_005368.2| AACCCCAAATCCAAGCCATTAACTACACCTGACAGTAGCAATTGTCTGATTAATCACTGG
gi|44955884|ref|NM_203377.1| AACCCCAAATCCAAGCCATTAACTACACCTGACAGTAGCAATTGTCTGATTAATCACTGG
 ************************************************************
 
gi|44955887|ref|NM_203378.1| CCCCTTGAAGACAGCAGAATGTCCCTTTGCAATGAGGAGGAGATCTGGGCTGGGCGGGCC
gi|44955876|ref|NM_005368.2| CCCCTTGAAGACAGCAGAATGTCCCTTTGCAATGAGGAGGAGATCTGGGCTGGGCGGGCC
gi|44955884|ref|NM_203377.1| CCCCTTGAAGACAGCAGAATGTCCCTTTGCAATGAGGAGGAGATCTGGGCTGGGCGGGCC
 ************************************************************
 
gi|44955887|ref|NM_203378.1| AGCTGGGGAAGCATTTGACTATCTGGAACTTGTGTGTGCCTCCTCAGGTATGGCAGTGAC
gi|44955876|ref|NM_005368.2| AGCTGGGGAAGCATTTGACTATCTGGAACTTGTGTGTGCCTCCTCAGGTATGGCAGTGAC
gi|44955884|ref|NM_203377.1| AGCTGGGGAAGCATTTGACTATCTGGAACTTGTGTGTGCCTCCTCAGGTATGGCAGTGAC
 ************************************************************
 
gi|44955887|ref|NM_203378.1| TCACCTGGTTTTAATAAAACAACCTGCAACATCTCA
gi|44955876|ref|NM_005368.2| TCACCTGGTTTTAATAAAACAACCTGCAACATCTCA
gi|44955884|ref|NM_203377.1| TCACCTGGTTTTAATAAAACAACCTGCAACATCTCA
 ************************************

The three DNA sequences encode the identical myoglobin protein, as follows:

 
>gi|4885477|ref|NP_005359.1| myoglobin [Homo sapiens]
MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVL
TALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAQGAMNKALELFR
KDMASNYKELGFQG
 
>gi|44955885|ref|NP_976311.1| myoglobin [Homo sapiens]
MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVL
TALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAQGAMNKALELFR
KDMASNYKELGFQG
 
>gi|44955888|ref|NP_976312.1| myoglobin [Homo sapiens]
MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVL
TALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAQGAMNKALELFR
KDMASNYKELGFQG